Abstract: The problems of heavy ultrasonic attenuation, varied sound velocity, and waveform distortion in bone tissue lead to weak signal and inaccurate imaging in commercial ultrasound devices for bone measurement. The purpose of this article is to develop a portable ultrasound imaging device suitable for bone evaluation. A 64-channel ultrasound imaging device was designed based on programmable logic hardware (FPGA). The emission voltage of the instrument is increased using a reactive boosting and stabilizing method, and a multi-stage programmable amplification circuit is used to increase the gain of the received signals in each channel. The bone ultrasound imaging device has a dimension of 90 mm (length) × 55 mm (width) × 30 mm (height). The device is weighted as 120 g and has good portability. The maximum peak to peak emission voltage of each channel is 180 V, and the maximum gain of each channel is 48 dB. The instrument integrates plane wave imaging and phase transfer migration imaging methods to perform ultrasound imaging on ex vivo bone samples of different thicknesses, and in vivo experiments were conducted on the tibia of volunteers. The results showed that the non-uniform distribution of the speed of sound causes the inaccurate display of bone thickness using the planar wave imaging, resulting in significant measurement errors (the relative error is 51.5%). Phase shift migration imaging based on sound velocity model can accurately display bone thickness with a relative error of 6.8%. In vivo measurements on human tibia showed that, compared with commercial instrument, the custom-made instrument could provide ultrasonic images clearly showing the bone boundaries of tibia with relatively high accuracy in the measurement of tibia thickness. The bone ultrasound imaging instrument developed in this study has good portability and scalability, which can accurately image bone thickness and contribute to the accurate diagnosis of bone diseases such as osteoporosis.